目的 建立测定大鼠血浆中新人参二醇(NPD)浓度的UPLC-MS/MS检测方法,研究NPD的大鼠血浆蛋白结合率。方法 在体外采用平衡透析法模拟NPD体内与血浆蛋白结合的过程,通过UPLC-MS/MS分析测定不同浓度NPD在透析袋内血浆中和透析外缓冲液中的浓度,计算NPD的大鼠血浆蛋白结合率。结果 NPD在0.05~8 μg·mL-1内呈现良好的线性关系,质控样品的日内和日间精密度(RSD)均小于15%,方法回收率均高于79.37%;NPD在低、中、高3个浓度水平下的大鼠血浆蛋白结合率分别为(86.55±4.50)%、(76.50±2.61)%和(78.25±1.32)%,各浓度间的结合率数据无显著性差异(P>0.05)。结论 NPD在大鼠体内主要以与血浆蛋白结合的形式存在。
Abstract
OBJECTIVE To develop an UPLC-MS/MS method for the determination of neopanaxadiol (NPD) in rat plasma sample, and to investigate plasma protein binding rate of NPD in rats. METHODS Under three different concentrations, equilibrium dialysis method was utilized to imitate the binding process between NPD and plasma protein. The concentration of NPD in and out of the dialysis membrane was determined by UPLC-MS/MS and the plasma protein binding rate of NPD were calculated. RESULTS Excellent linearity was found between 0.05-8 μg·mL-1. Intra- and inter-day precision values (RSD) of QC samples were both below 15% and the extraction recoveries of NPD from biological matrices were better than 79.37%. The plasma protein binding rates of NPD were (86.55±4.50)%、(76.50±2.61)% and (78.25±1.32)% at low, middle and high concentrations, respectively. There was no significant difference among three concentrations. CONCLUSION These results indicate the high plasma protein binding rates of NPD in plasma in combination mode.
关键词
超高效液相色谱-串联质谱法 /
新人参二醇 /
血浆蛋白结合率 /
平衡透析法
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Key words
UPLC-MS/MS /
neopanaxadiol (NPD) /
plasma protein binding rate /
equilibrium dialysis
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中图分类号：
R969
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参考文献
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脚注
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基金
国家自然科学基金资助项目(31070308);辽宁省教育厅科学研究项目(L2015141)
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